ABSTRACT
No abstract available.
Subject(s)
Aged , Female , Humans , Acute Disease , Anti-Bacterial Agents/therapeutic use , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Buttocks , Debridement , Diabetes Mellitus, Type 2/complications , Escherichia coli Infections/diagnosis , Microbial Sensitivity Tests , Muscle, Skeletal/microbiology , Pyelonephritis/diagnosis , Pyomyositis/diagnosis , Tomography, X-Ray Computed , Treatment OutcomeABSTRACT
OBJECTIVE@#To study the relationship between the changes of microbial ATP in rat muscle tissue and the postmortem interval (PMI).@*METHODS@#Healthy SD rats were sacrificed and their muscles were sampled at different postmortem intervals. The concentration of microbial ATP was detected using bioluminescent assay and the data was statistically analyzed.@*RESULTS@#The content of microbial ATP in rat muscle tissue increased along with PMI extension and peaked on postmortem day 7, thereafter decreased gradually, but increased slightly on postmortem day 10 once again. The PMI correlated best with the content of microbial ATP in rat muscle tissue within 9 days. If the PMI was the independent variable, the cubic polynomial regression equation was y = 0.020x(3) - 0.166x(2) - 0.666x + 13.412 (r2 = 0.989, P < 0.01).@*CONCLUSION@#Changes of microbial ATP content in rat muscle tissue may be used for estimation of PMI. Since this assay requires only small amount of tissue with much less influence by self-decomposition, it may broaden the time range of PMI estimation.
Subject(s)
Animals , Female , Male , Rats , Adenosine Triphosphate/metabolism , Forensic Pathology/methods , Muscle, Skeletal/microbiology , Postmortem Changes , Random Allocation , Rats, Sprague-Dawley , Time FactorsABSTRACT
Paralingual Space is a region located on the floor of the oral cavity. The cavity has great importance in communications and for the spread of infectious diseases. This study investigated the level of dissemination of 0.2 ml of an oily pigment solution, into paralingual space of 20 heads of male Sprague Dawley rats. The results can be seen by the spread of the solution in the three segments of paralingual space, being higher in the middle third of the region, although the differences were not significant (p<0.05). With these observations, the discussion is about the possibility that an infection may spread into paralingual space opposite the muscles of the medial wall of this space.
El espacio paralingual es una región ubicada en el piso de la cavidad oral con comunicaciones de gran importancia para la difusión de procesos infecciosos. El presente estudio investigó el nivel de difusión de 0,2 ml de una solución oleosa pigmentada, hacia el espacio paralingual opuesto en 20 cabezas de ratas Sprague Dawley. En los resultados obtenidos se observa la difusión de la solución en los tres segmentos del espacio paralingual, siendo mayor en el tercio medio de la región, aunque las diferencias no resultaron significativas (p<0,05). Con estas observaciones se discute acerca de la posibilidad de que un proceso infeccioso pueda difundir hacia el espacio paralingual opuesto a través de la musculatura de la pared medial del espacio.